Professor, Department of Pathology, Sackler Faculty of Medicine
פרופ' כורת הירשברג
סגל אקדמי בכיר בפתולוגיה
פתולוגיה
סגל אקדמי בכיר
Positions
קורות חיים
Professor Koret Hirschberg is a cell biologist at Tel Aviv University Medical School, specializing in live cell imaging and intracellular trafficking. A graduate of the Weizmann Institute, he further developed his expertise during his postdoctoral training at the NIH with Jennifer Lippincott-Schwartz. His research has led to a significant dogma shift in understanding ER-to-Golgi transport, advancing knowledge of how proteins move through the secretory pathway. His interdisciplinary approach combines cell biology, physics, and mathematical modelling to analyse vesicle dynamics, membrane domain organization, and protein-lipid interactions. This integrative method has uncovered vital insights into membrane compartmentalization and cellular organization. Professor Hirschberg's work bridges fundamental cellular processes with biophysical principles, contributing to our understanding of membrane dynamics and intracellular trafficking. His work continues to shape our understanding of cellular transport and its role in health and disease.
תחומי מחקר
Molecular membrane biology
Intracellular trafficking
Live cell imaging
פרסומים
SCIENTIFIC PUBLICATIONS
1. Hermoso, T., Fishelson, Z. , Becker S. I ., Hirschberg K. , and Jaffe C. L.
Leishmanial protein kinases phosphorylate components of the complement system.
EMBO J. 10:4061-7. 1991.
IF=9.205, 21/288 (Biochemistry) Times cited: 134
2. Hirschberg K. , Rodger J. and Futerman A. H.
The long-chain sphingoid base of sphingolipids is acylated at the cytosolic surface of the endoplasmic reticulum in rat liver.
Biochem. J. 290:751-757. 1993.
IF=3.579, 61/289 (Biochemistry) Times cited: 228
3. Schwarz A. , Rapaport E. , Hirschberg K. and Futerman A. H.
A regulatory role for sphingolipids in neuronal growth - Inhibition of sphingolipid synthesis and degradation have opposite effects on axonal branching.
J. Biol. Chem. 270:10990-10998. 1995.
IF=4.77, 66/289 (Biochemistry) Times cited: 205
4. Futerman, A. H. , Hirschberg K. , Meivar-Levy I. , Rapaport E. , Schwarz A. , Sofer A. and Zisling R.
Vesicle transport during cell growth and in the maintenance of cell polarity.
Biochem. Soc. Trans. 23:530-534. 1995.
IF=3.71, 94/289 (Biochemistry) Times cited: 4
5. Hirschberg, K. , Zisling R. , van-Echten-Deckert G. and Futerman A. H.
Ganglioside synthesis during the development of neuronal polarity: major changes occur during axonogenesis and axon elongation, but not during dendrite growth or during synaptogenesis.
J. Biol. Chem. 271:14876-14882. 1996.
IF=4.77, 66/289 (Cell Biology) Times cited: 86
6. Presley, J. F. , Cole N. B. , Schroer T. A. , Hirschberg K. , Zaal K. J. M. and Lippincott-Schwartz J.
ER-to-Golgi transport visualized in living cells.
Nature. 389:81-5. 1997.
IF=36.28, 11/8281 (?) Times cited: 1372
7. Presley, J. F. , Smith C. , Hirschberg K. , Cole N. B. , Zaal K. J. M. , Miller C. M. and Lippincott-Schwartz J.
Golgi membrane dynamics.
Mol. Biol. Cell. 9:1617-1626. 1998.
IF=4.948, 53/180; Times cited: 107
8. Hirschberg K. , Miller C. M. , Ellenberg J. , Presley J. F. , Siggia E. D. , Phair R. D. , and Lippincott-Schwartz J.
Kinetic analysis of secretory protein trafficking and characterization of Golgi to plasma membrane transport intermediates in living cells.
J. Cell Biol. 143; 6, 1485-1503. 1998.
IF=10.26, 16/180 (Cell Biology) Times cited: 657
9. Hirschberg, K. , and Lippincott-Schwartz J.o
Secretory pathway kinetics and in vivo analysis of protein traffic from the Golgi complex to the cell surface.
FASEB J. 13: 251-256. 1999.
IF=5.71, 39/180 (Cell Biology) Times cited: 48
10. Zaal, K. , Smith, C.L. , Polishchuk, R.S. , Altan, N. , Cole, N. , Ellenberg, J. , Hirschberg,
K. , Presley, J. , Roberts, T. , Siggia, E. , Phair, R. , and Lippincott-Schwartz, J.
Golgi membranes are absorbed into and re-emerge from the ER during mitosis.
Cell 99, 589-601. 1999.
IF=32.4, 2/180 (Cell Biology) Times cited: 373
11. Nichols B. J. , Kenworthy A. K. , Polishchuk R. S. , Lodge R. , Roberts T. H. , Hirschberg
K. , Phair R.D. and Lippincott-Schwartz J.
Rapid cycling of lipid raft markers between the cell surface and Golgi complex.
J.Cell Biol. 153;3, 529-541. 2001.
IF=10.26, 16/180 (Cell Biology) Times cited: 640
12. Ward T. H. , Polishchuk R. S. , Caplan S. , Hirschberg K., and Lippincott-Schwartz J.
Maintenance of Golgi structure and function depends on the integrity of ER export
J. Cell Biol. 155; 557-570. 2001.
IF=10.26, 16/180 (Cell Biology) Times cited: 462
Recommended by Faculty of 1000 (3.0): http://www.f1000biology.com/article/id/1003285
13. Rotman-Pikielny P. *, Hirschberg K. ,* Maruvada P., Suzuki K. , Royaux I. E. , Green E. D. , Kohn L. D. , Lippincott-Schwartz J. , and Yen P. M.
Retention of Pendrin in the Endoplasmic Reticulum is a Major Mechanism for Pendred Syndrome.
Human Molecular Genetics. Volume 11;21. 2625-33. 2002.
*- co first authors
IF=7.81, 12/132 (Genetics) Times cited: 109
14. Barzilay E. , Ben-Califa N. , Supino-Rosin L., Kashman Y., Hirschberg K. and
Neumann D.
Geldanamycin-associated inhibition of intracellular trafficking is attributed to a co-purified Activity.
J. Biol. Chem. 279:6847-52. 2004
IF=4.77, 66/289 (Cell Biology) Times cited: 18
15. Kogan T. V., Jadoun J. , Hirschberg K., Mittelman L. , Osherov N.
J. Infect. Dis. 189: 1965-73. 2004
IF=6.4, 3/70 Times cited: 126
16. Shepshelovich J. , Goldstein-Magal L., Globerson A., M. Yen P., Rotman-Pikielny P. and Hirschberg K.
Protein-synthesis inhibitors and the chemical chaperone TMAO reverse ER perturbation induced by overexpression of the iodide transporter pendrin.
J. Cell science. 118, 1577-1586. 2005
IF=6.1, 38/180 (Cell Biology) Times cited: 54
17. Barzilay E., Ben-Califa N., Hirschberg K.* and Neumann N.*
Uncoupling of Brefeldin A-Mediated COP-I Dissociation from Golgi Redistribution to the Endoplasmic Reticulum.
Traffic. 6, 794-802. 2005
IF=4.92, 55/180 (Cell Biology) Times cited: 28
*- co-senior authors
18. Kanaan M., Walsh T., Abu Rayan A., Abu Sa’ed J., Shahin H., Lee M. K., Shepshelovich J., Hirschberg K., Tekin M., Avraham K. B. and Claire King M..
Genomic Analysis of a Heterogeneous Mendelian Phenotype: Multiple Novel Alleles For Inherited Hearing Loss In The Palestinian Population
Human Genomics. 2, 203-11. 2006.
IF= Not Indexed; Times cited: 60
19. Dukhovny A., Goldstein Magal L. and Hirschberg K..
The MAL proteolipid restricts membrane pore expansion and percolation during cold differential extraction with Triton X-100
Molecular Membrane Biology. 23, 245-57. 2006.
IF=2.86, 102/180 (?); Times cited: 12
20. Kapp-Barnea Y., Ninio-Many L., Hirschberg K., MFukuda M., Jeromin A., and Sagi-Eisenberg R..
Neuronal Calcium Sensor-1 and Phosphatidylinositol 4-Kinase Stimulate Extracellular Signal-regulated Kinase 1/2 Signaling by Accelerating Recycling through the Endocytic Recycling Compartment.
Mol Biol Cell. 17, 4130-4141. 2006
IF=4.948, 53/180; Times cited: 27
21. Fass E., Shvets E., Degani I., Hirschberg K., and Elazar Z.
Microtubules support production of starvation-induced autophagosomes but not their targeting and fusion with lysosomes.
J. Biol. Chem. 281,36303-36316 2006.
IF=4.77, 66/289 (Cell Biology) Times cited: 330
22. Vasserman G., Goldstein Magal L., Shepshelovich J., Elifaz E. and Hirschberg K..
Processing of the VSVG protein is not a rate-limiting step for its efflux from the Golgi complex.
Biochem Biophys Res Commun. 351, 689-94. 2006.
IF=2.48, 173/289 Times cited: 6
23. Haberman Y., Ziv I., Gorzalczany Y., Hirschberg K., Mittleman L., Fukuda M. and Sagi-Eisenberg R..
Synaptotagmin (Syt) IX is an Essential Determinant for Protein Sorting to Secretory Granules in Mast Cells. Blood 109(8), 3385-92. 2006.
IF=9.89, 2/68 (Cell Biology) Times cited: 19
24. Shapira I., Charuvi D., Elkabetz Y., Hirschberg K. and Bar-Nun S..
Distinguishing between retention signals and degrons acting in ERAD
J. Cell Science. 120, 4377-87 2007.
IF=6.1, 38/180 (Cell Biology); Times cited: 15
25. Brownstein Z. N. , Dror A. A. , Gilony D. , Migirov L, Hirschberg K. and Avraham K.B.
A Novel PDS Deafness Mutation is Retained in the Endoplasmic Reticulum
Otolaryngology, Head & Neck.134, 403-7 2008.
IF=1.72, 70/198 (Cell Biology) Times cited: 18
26. Nahari T., Barzilay E., Hirschberg K. and Neumann D.
A transplanted NPVY sequence in the cytosolic domain of the erythropoietin receptor enhances maturation.
Biochem. J. 410, 409-16. 2007
IF=1.72, 61/289 (Cell Biology); Times cited: 5
27. Dukhovny A., Papadopulos A. and Hirschberg K.
Quantitative live-cell analysis of cargo-protein sorting in the ER
J. Cell Science. 121, 865-76 2008
IF=6.1, 38/180 (Cell Biology); Times cited: 28
28 Shaharabany M., Holtzman E. J., Mayan H., Hirschberg K., Seger R. and Farfel Z.
Distinct pathways for the in the signaling of hypertonicity
FEBS J. 275, 1631-42 2008.
IF=3.79, 89/289 (Cell Biology) Times cited: 26
29 Patterson G. H.*, Hirschberg K.*, Polishchuk R. S., Gerlich D.,Phair R. D., and Lippincott-Schwartz J..
Transport through the Golgi apparatus by rapid partitioning within a two-phase membrane system.
Cell. 133, 1055-1067 2008.
IF=32.4, 2/180 (Cell Biology); Times cited: 310
*- Equal contributors. Recommended by Faculty of 1000 (8.4): http://www.f1000biology.com/article/id/1116078
30 Shaklai S., Somech R., Gal-Yam E. N., Deshet-Unger N., Moshitch-Moshkovitz S., Hirschberg K., Amariglio N., Simon A. J., Rechavi G.
LAP2 binds BAF and suppresses LAP2-mediated transcriptional repression.
Eur J Cell Biol. 87, 267-78 2008.
IF=32.4, 85/180 (Cell Biology); Times cited: 16
31 Peretti D., Dahan N., Shimoni E., Hirschberg K., Lev S..
Coordinated lipid transfer between the endoplasmic reticulum and the Golgi complex requires the VAP proteins and is essential for Golgi-mediated transport.
Mol. Biol. Cell. 19, 3871-84 2008.
IF=4.948, 53/180 (Cell Biology); Times cited: 344
Recommended by Faculty of 1000 (3.0): http://www.f1000biology.com/article/id/1118855
32 Dukhovny A., Yaffe Y., Shepshelovich J. and Hirschberg K.
The length of cargo-protein transmembrane segments drives secretory transport by facilitating cargo concentration in export domains.
J. Cell Science. 122, 1759-67 2009
IF: 6.779, 28/156( Cell Biology) Times cited: 36
33. Goldstein Magal L., Yaffe Y., Shepshelovich J. , Aranda J. F., del Carmen de Marco M., Gaus K., Alonso M. A. andHirschberg K.
Clustering and lateral concentration of raft lipids by the MAL protein.
Mol. Biol. Cell. 16, 3751-62 2009
IF=6.1; Rank: 38/180; Times cited: 61
34 Wagner V., Stadelmeyer E., Riederer M., Regitnig P., Gorischek A., DeVvaney T., Schmidt K., Tritthart H. A., Hirschberg K., Bauernhofer T.( C), and Schreibmayer W..
Cloning and Characterization of GIRK1 variants Resulting from Alternative RNA Editing of the KCNJ3 Gene Transcript in a Human Breast Cancer Cell Line.
J Cell Biochem.110, 598-608. 2010
IF=2.057; Rank: 131/180; Times cited: 33
35 Alfaguter-Azoulay I., Yaffe Y., Licht-Murava A., Urbanska M., Jaworski J., Pietrokovski S., Hirschberg K. and Eldar-Finkelman H.
Distinct molecular regulation of GSK-3alpha isozyme controlled by its N-terminal region. Functional role in calcium/calpain signaling
J. of Biol. Chem. 286, 15, 13470-80. 2011.
IF=4.77; Rank: 66/289; Times cited: 53
36 Benjamin S., Weidberg H., Rapaport D., Pekar O., Nudelman M., Segal D., Hirschberg K., Katzav S., Ehrlich M. and Horowitz M.. EHD2 mediates trafficking from the plasma membrane by modulating Rac1 activity.
Biochemical J. 439, 3, 433-42. 2011
IF 4.77, 66/289 (Cell Biology); Times cited: 44
37 Yaffe Y., Shepshelovitch J. , Yeheskel A., Shmerling H., Kwiatek J. M., Gaus K., Pasmanik-Chor M., and Hirschberg K..
The MARVEL transmembrane domain of Occludin mediates oligomerization and targeting to the basolateral surface in epithelia. J. Cell Science. 125, 3545-3556. 2012
IF: 6.779, 28/156 (Cell Biology) Times cited: 41
38 Nevo-Yassaf I., Yaffe Y., Asher M., Ravid O., Eizenberg S.Henis Y. I., Nahmias Y., Hirschberg K., and Sklan E. H.. A role forTBC1D20 and Rab1 in Hepatitis C virus replication via interaction with LD bound NS5A. J Virol. 86, 6491-6502. 2012
IF 5.4, 5/33 (Virology) Times cited: 55
39 David N., Yaffe Y., Hagoel L., Elazar M., Glenn J. S., Hirschberg K., H. Sklan E.H.. The interaction between the Hepatitis C proteins NS4B and NS5A is involved in viral replication. Virology. 475, 139-49. 2015
IF 5.4, 16/33 (Virology) Times cited: 21
40 Yaffe Y., Hagger I. , Nevo Yassaf I., Shepshelovitch J., Sklan E. H., Elkabetz Y., Yeheskel A., Pasmanik-Chor M.( C),Benzing C., Macmillan A., Gaus K., Eshed-Eisenbach Y., Peles E., and Hirschberg K.
The myelin proteolipid Plasmolipin, forms oligomers and induces liquid ordered membranes in the Golgi apparatus. 128, 2293-302 J. Cell Science. 2015
IF 4.706, 48/187 (Cell Biology) Times cited: 24
41 Skalka N.,Caspi M., Lahav-Ariel L., Loh Y.P., Hirschberg K., and Rosin-Arbesfeld R.
Carboxypeptidase E (CPE) inhibits the secretion and activity of Wnt3a.
Oncogene 35, 6416-28. 2016.
IF=7.519 26/290 Q2 (Oncology) Times cited: 10
42. Yonemura Y., Li X., Muller K., Kramer A., Atigbire P., Mentrup T., Feuerhake T., Kroll T., Shomron O., Nohl R., Arndt H.D., Hoischen C., Hemmerich P., Hirschberg K., Kaether C., Inhibition of cargo export at ER exit sites and the trans-Golgi network by the secretion inhibitor FLI-06, J Cell Sci 129 3868-77. 2016
*- co-coresponding author
IF=4.431 61/190 Q2 (Cell Biology) Times cited: 17
43. Nevo-Yassaf I, lovelle M., Nahmias Y., Hirschberg K *, Sklan E. H.* Methods. 127,30-36. 2017. Live cell Imaging andanalysis of lipid droplets biogenesis in HCV infected cells.
*- co-coresponding author
IF=3.812 12/79 (Biochemical Research Methods) Times cited: 8
44. O. Klein, A. Roded, N. Zur, N. P. Azouz, O. Pasternak, K. Hirschberg, I.
Hammel, P. A. Roche, A. Yatsu, M. Fukuda, S. J. Galli and R. Sagi- Eisenberg. Scientific Reports 7; 15315.. 2017. Rab5 is critical for Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis.
IF=3.998 17/71 (Multidisciplinary Sciences) Times cited: 18
45. Klein, O., Roded, A., Hirschberg, K., Fukuda, M., Galli, S. J., Sagi-Eisenberg, R. J Vis Exp. 136. 2018. Imaging FITC-dextran as a Reporter for Regulated Exocytosis.
IF=1.163 45/71 Q3 (Interdisciplinary Sciences) Times cited: 7
46. Shomron O, Hirschberg K, Burakov A, Kamentseva R, Kornilova E, Nadezhdina E, Brodsky I. Traffic. 22, 64-77. 2021. Positioning of endoplasmic reticulum exit sites around the Golgi depends on BicaudalD2 and Rab6 activity.
IF=4.045 82/195 Q2 (Cell Biology) Times cited: 4
47. Shomron O., Nevo-Yassaf I., Aviad T., Yaffe Y., Eitan Erez Zahavi E. E., Dukhovny A., Eran Perlson E., Brodsky I., Adva Yeheskel A., Pasmanik-Chor M., Mironov, A., Beznoussenko G. V., Mironov A.A., Ella H. Sklan E. H., Patterson G. H., Yonemura Y., Sannai M., Kaether C., and Hirschberg K. J. Cell Biol. 220, 6. 2021COPII collar defines the boundary between ER and ER Exit Site and does not coat cargo containers.
IF=8.81 26/195 Q1 (Cell Biology) Times cited: 38
48. Moalem Y, Malis Y, Voloshin K, Dukhovny A, Hirschberg K, Sklan E H. Frontiers in Immunology Sandfly fever viruses attenuate the type I interferon response by targeting the phosphorylation of JAK-STAT components 13, 865797. 2022.. Times cited: 1
49. Evron-Levy T, Caspi M, Wittenstein A, Shorer-Arbel Y, Shomron O, Hirschberg K, Kariv R, Rosin-Arbesfeld R. Adenoma-Derived Organoids for Precision Therapy. Organoids, 1, 54-68. 2022. Times cited: 1
50. Werner, H., Abou Kandil, A., Meir, Z., Malis, Y., Shadkchan, Y., Masrati, G., Ben-Tal, N., Hirschberg, K., and Osherov, N. (2023). Identification and Analysis of Fungal-Specific Regions in the Aspergillus fumigatus Cu Exporter CrpA That Are Essential for Cu Resistance but Not for Virulence. Int J Mol Sci 24. 10.3390/ijms24043705.
51. Armoza-Eilat, S., Y. Malis, M. Caspi, R. Tarabe, O. Shomron, K. Hirschberg, and R. Rosin-Arbesfeld. 2023. The C-terminal Amphipathic Helix of Carboxypeptidase E Mediates Export from the ER and Secretion via Lysosomes. J Mol Biol. 435:168171.
52. Malis, Y., S. Armoza-Eilat, I. Nevo-Yassaf, A. Dukhovny, E.H. Sklan, and K. Hirschberg. 2024. Rab1b facilitates lipid droplet growth by ER-to-lipid droplet targeting of DGAT2. Science Advances.10: eade7753.
53. Malis Y, Gaash D, Masri R, Moalem Y, Hirschberg G M, George H. Patterson G H and Hirschberg H. Using Photoswitching FRET to Define the Interaction Boundaries of Rab1b GTPase and Secretory Cargo. In preparation for resubmission to J. Cell Biol.
REVIEW ARTICLES
1 Lippincott-Schwartz J. , Roberts T. H. and Hirschberg K.
Secretory Protein trafficking and organelle dynamics in living cells.
Ann. Rev. of cell and dev. Biol. 16; 557-589. 2000.
IF=20.241 85/180 (Cell Biology), Times cited: 597
2 Malis Y., Hirschberg K., Kaether C. Hanging the coat on a collar: Same function but different localization and mechanism for COPII.
BioEssays. 44, 2200064 2022 Times cited: 2
3 Farhan H, Raote I, Campelo F, Ge L, Hirschberg K, Forrester A, Zanetti G, Lippincott-Schwartz J, Pastor-Pareja J C, Perez F, Kota Saito K, Malhotra V. ER exit sites: A roadmap for membrane trafficking research. Nature Reviews Molecular Cell Biology. In press
CHAPTERS IN BOOKS
1 Lippincott-Schwartz, J. Presley, J. F. Zaal, K. Hirschberg , C. M. Miller and J. Ellenberg. Monitoring the dynamics and mobility of membrane proteins tagged with green fluorescent protein.
Methods in Cell Biology volume 58 green fluorescent protein pp 261-280.
Edited by K. Sullivan and S. Kay. Academic Press, San Diego, USA. 1998.
Times cited: 82
2 Hirschberg, K. , Phair R. D. and Lippincott-Schwartz J.
Kinetic Analysis of Intracellular Trafficking using VSVG-GFP in Single Living Cells. Chimeric Genes and Proteins Edited by S. D. Emr, J. Thorner, and J. Abelson. Academic Press.
Methods in Enzymology. 327B, 69-89. 1999.
IF=2.194 42/78 (Biochemical research methods) Times cited: 26
3 Shepshelovich J. and Hirschberg K.
Expression, localization and topology of fluorescently tagged PM-targeted transmembrane proteins in Protein Targeting Protocols Edited by Mark van der Giezen. Humana Press. 390, 26, 393-403.2007
