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National Laboratory for the Genetics
of Israeli Populations

DNA samples and B-lymphoblastoid cell lines availability

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You may order either DNA samples or matching human B-lymphoblastoid cell lines.

Note: Each DNA sample in our collection has a matching cell line. For example, you may first order a large set of DNA samples, and later order the desired matching cell lines from the same donors.

• The price for each growing cell line is US $150 (excluding shipping charges).
• The price for each 10 ug DNA sample is US $45 (excluding shipping charges).
• The price for each 5 ug DNA sample is US $30 (excluding shipping charges).
  
• Our DNA samples are sent in sterile distilled water at a concentration of ~500 ng/ul and are PCR-ready. 
• Each 5 ug DNA sample is sufficient for approximately 50 to 100 PCR reactions. 
  
• After receiving an order for cell lines one vial of each frozen cell line is thawed. 
• The viability of the cells and lack of mycoplasma contamination is checked by our staff. 
• B-cell lines are viable for at least one week at ambient temperatures, and are shipped as growing cultures (approximately 20 million cells).in a volume of 15 ml RPMI.
• Shipment of frozen vials in dry-ice is not recommended due to possible delays by local customs.
  
  
• To place an order, please print, complete and sign the order form and fax along with an
  Institutional Purchase Order to ++972-3-640-5168 or email a scanned PDF file to gurwitz@post.tau.ac.il
• The original forms should be mailed to the address shown below.
• Please include a short cover letter explaining the research project for which the DNA samples or cell lines will be used.
  
  
• Minimal orders are US$ 500
• Special discounts are available for orders exceeding US $5,000 (please inquire) 
• Kindly contact us before placing an Institutional Purchase Order (contact details below), as some DNA samples could be temporarily out of stock.
  
  
• Orders not accompanied by an Institutional Purchase Order will not be processed. 
• Shipments are made with courier within 2 - 3 weeks of receiving your order.
• Israeli customers: please note that there is no VAT 

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DNA Samples and Cell Line Catalog All donors are healthy adults (A matching B-lymphoblastoid cell line is available for each DNA sample)
Ethnic Group	Number of unrelated donors
Jewish
· Ashkenazi  (Central European ancestry)	466
· Ethiopian	72
· Georgian	24
· Iranian	76
· Iraqi	103
· Kuchin (India)	85
· Libyan	89
· Moroccan	150
· Sephardi (Turkey & Bulgaria )	166
· Tunisian	29
· Yemenite	159
Bedouin	58
Druze	79
Palestinian	117

#  Distribution of some of the DNA samples and cell lines marked by # may require prior agreement of the researchers who donated the samples to our repository. Please check with us before placing an order for these samples. 

Some notes about our donors

· All donors are adult Israeli citizens (over 18 years old) and have given written informed consent for the study of their genetic material (DNA or cells) for biomedical research.

· Commercial use of samples is not allowed.

· Ethnicity is self-defined by the donors.

· For Jewish donors, it is defined according to the place of birth of their four grandparents. For example, Iraqi Jewish donors have four grandparents who were born in Iraq .

· Gender and age are available for all donors (with some exceptions for age).

· Height and weight and smoking status (smoker / non-smoker) are available for some donors.

Some examples for studies using our DNA samples or cell lines

• Counting the founders: the matrilineal genetic ancestry of the Jewish Diaspora. Behar DM, Metspalu E, Kivisild T, Rosset S, Tzur S, Hadid Y, Yudkovsky G, Rosengarten D, Pereira L, Amorim A, Kutuev I, Gurwitz D, Bonne-Tamir B, Villems R, Skorecki K. PLoS ONE. 2008 Apr 30;3(4):e2062.
• Ethnic differences in the VKORC1 gene polymorphism and an association with warfarin dosage requirements in cardiovascular surgery patients. Nakai K, Tsuboi J, Okabayashi H, Fukuhiro Y, Oka T, Habano W, Fukushima N, Nakai K, Obara W, Fujioka T, Suwabe A, Gurwitz D. Pharmacogenomics. 2007 Jul;8(7):713-9.
• Ethnicity-related polymorphisms and haplotypes in the human ABCB1 gene. Kimchi-Sarfaty C, Marple AH, Shinar S, Kimchi AM, Scavo D, Roma MI, Kim IW, Jones A, Arora M, Gribar J, Gurwitz D, Gottesman MM. Pharmacogenomics. 2007 Jan;8(1):29-39.
• Chromosomal mapping and phenotypic characterization of hereditary otosclerosis linked to the OTSC4 locus. Brownstein Z, Goldfarb A, Levi H, Frydman M, Avraham KB.Arch Otolaryngol Head Neck Surg. 2006 Apr;132(4):416-24.
• Behar DM, Hammer MF, Garrigan D, Villems R, Bonne-Tamir B, Richards M, Gurwitz D, Rosengarten D, Kaplan M, Della Pergola S, Quintana-Murci L, Skorecki K. MtDNA evidence for a genetic bottleneck in the early history of the Ashkenazi Jewish population. Eur J Hum Genet. 2004;12:355-364.
• Mitra N, Ye TZ, Smith A, Chuai S, Kirchhoff T, Peterlongo P, Nafa K, Phillips MS, Offit K, Ellis NA. Localization of cancer susceptibility genes by genome-wide single-nucleotide polymorphism linkage-disequilibrium mapping. Cancer Res. 2004;64:8116-8125.
• Shen P, Lavi T, Kivisild T, Chou V, Sengun D, Gefel D, Shpirer I, Woolf E, Hillel J, Feldman MW, Oefner PJ. Reconstruction of patrilineages and matrilineages of Samaritans and other Israeli populations from Y-chromosome and mitochondrial DNA sequence variation. Hum Mutat. 2004;24:248-260.
• Rosenmann H, Meiner Z, Dresner-Pollak R, Kahana E, Aladjem Z, Grenader T, Wertman E, Abramsky O. Lack of association of interleukin-1beta polymorphism with Alzheimer's disease in the Jewish population. Neurosci Lett. 2004;363:131-133.
• Picornell A, Jimenez G, Castro JA, Ramon MM. Minimal Y-chromosome haplotypes plus DYS287 in Jewish populations. J Forensic Sci. 2004;49:410-412.
• Luo HR, Aloumanis V, Lin KM, Gurwitz D, Wan YJ. Polymorphisms of CYP2C19 and CYP2D6 in Israeli ethnic groups. Am J Pharmacogenomics. 2004;4:395-401.
• Nakai K, Habano W, Nakai K, Fukushima N, Fujita T, Gurwitz D. Ethnic differences of coronary artery disease-associated SNPs in two Israeli healthy populations using MALDI-TOF mass spectrometry. Life Sci. 2004;75:1003-1010.
• Gilad Y, Lancet D. Population differences in the human functional olfactory repertoire.
  Mol Biol Evol. 2003; 20:307-314.
• Hammer MF, Blackmer F, Garrigan D, Nachman MW, Wilder JA. Human population structure and its effects on sampling Y chromosome sequence variation. Genetics. 2003;164:1495-1509.
• Menashe I, Man O, Lancet D, Gilad Y. Population differences in haplotype structure within a human olfactory receptor gene cluster. Hum Mol Genet. 2002;11:1381-1390.
• Saunders MA, Hammer MF, Nachman MW. Nucleotide variability at G6pd and the signature of malarial selection in humans. Genetics. 2002;162:1849-1861.
• Foulkes WD, Thiffault I, Gruber SB, Horwitz M, Hamel N, Lee C, Shia J, Markowitz A, Figer A, Friedman E, Farber D, Greenwood CM, Bonner JD, Nafa K, Walsh T, Marcus V, Tomsho L, Gebert J, Macrae FA, Gaff CL, Paillerets BB, Gregersen PK, Weitzel JN, Gordon PH, MacNamara E, King MC, Hampel H, De La Chapelle A, Boyd J, Offit K, Rennert G, Chong G, Ellis NA. The founder mutation MSH2*1906G-->C is an important cause of hereditary nonpolyposis colorectal cancer in the Ashkenazi Jewish population. Am J Hum Genet. 2002;71:1395-1412.
• Gilad Y, Rosenberg S, Przeworski M, Lancet D, Skorecki K. Evidence for positive selection and population structure at the human MAO-A gene. Proc Natl Acad Sci U S A. 2002;99:862-867.
• Rosenberg NA, Woolf E, Pritchard JK, Schaap T, Gefel D, Shpirer I, Lavi U, Bonne-Tamir B, Hillel J, Feldman MW. Distinctive genetic signatures in the Libyan Jews. Proc Natl Acad Sci U S A. 2001;98:858-863.
• Mateu E, Calafell F, Lao O, Bonne-Tamir B, Kidd JR, Pakstis A, Kidd KK, Bertranpetit J. Worldwide genetic analysis of the CFTR region. Am J Hum Genet. 2001;68:103-117.
• Torroni A, Bandelt HJ, Macaulay V, Richards M, Cruciani F, Rengo C, Martinez-Cabrera V, Villems R, Kivisild T, Metspalu E, Parik J, Tolk HV, Tambets K, Forster P, Karger B, Francalacci P, Rudan P, Janicijevic B, Rickards O, Savontaus ML, Huoponen K, Laitinen V, Koivumaki S, Sykes B, Hickey E, Novelletto A, Moral P, Sellitto D, Coppa A, Al-Zaheri N, Santachiara-Benerecetti AS, Semino O, Scozzari R. A signal, from human mtDNA, of postglacial recolonization in Europe. Am J Hum Genet. 2001;69:844-852.
• Underhill PA, Shen P, Lin AA, Jin L, Passarino G, Yang WH, Kauffman E, Bonne-Tamir B, Bertranpetit J, Francalacci P, Ibrahim M, Jenkins T, Kidd JR, Mehdi SQ, Seielstad MT, Wells RS, Piazza A, Davis RW, Feldman MW, Cavalli-Sforza LL, Oefner PJ. Y chromosome sequence variation and the history of human populations. Nat Genet. 2000;26:358-361.
• Hammer MF, Redd AJ, Wood ET, Bonner MR, Jarjanazi H, Karafet T, Santachiara-Benerecetti S, Oppenheim A, Jobling MA, Jenkins T, Ostrer H, Bonne-Tamir B. Jewish and Middle Eastern non-Jewish populations share a common pool of Y-chromosome biallelic haplotypes.
  Proc Natl Acad Sci U S A. 2000;97:6769-6774.
• Kantor R, Gershoni JM. Distribution of the CCR5 gene 32-base pair deletion in Israeli ethnic groups. J Acquir Immune Defic Syndr
  Hum Retrovirol. 1999 Jan 1;20(1):81-4.

A list of over 100 published studies using our DNA samples is available upon request

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Contact:

David Gurwitz, Ph.D.

Director

National Laboratory for the Genetics of Israeli Populations

Department of Human Molecular Genetics & Biochemistry
Sackler Faculty of Medicine

Tel-Aviv University
Tel-Aviv 69978 Israel

Phone: ++972-3-640-7611 
Fax: ++972-3-640-5168 

E-mail: gurwitz@post.tau.ac.il

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